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Muscle fiber type in skeletal muscle biopsies collected from active and hibernating brown bears. Representative immunofluorescence images of muscle cross‐sections showing type I fibers (yellow/orange) and cell membranes (green) from the same animal in summer ( n = 5, summer) and winter ( n = 5, winter) are presented in A. The percentage of type I and type II fibers quantified in skeletal muscle biopsies from the same animals is shown in B, and the cross‐sectional area of type I and type II fibers is shown in C. Data are expressed as the mean with individual datapoints represented by dots (summer) and diamonds (winter), Red bars, summer; blue bars, winter.

Journal: Acta Physiologica (Oxford, England)

Article Title: The Preservation of Muscle Mitochondrial Machinery During Hypometabolic Hibernation in Scandinavian Brown Bears ( Ursus arctos )

doi: 10.1111/apha.70177

Figure Lengend Snippet: Muscle fiber type in skeletal muscle biopsies collected from active and hibernating brown bears. Representative immunofluorescence images of muscle cross‐sections showing type I fibers (yellow/orange) and cell membranes (green) from the same animal in summer ( n = 5, summer) and winter ( n = 5, winter) are presented in A. The percentage of type I and type II fibers quantified in skeletal muscle biopsies from the same animals is shown in B, and the cross‐sectional area of type I and type II fibers is shown in C. Data are expressed as the mean with individual datapoints represented by dots (summer) and diamonds (winter), Red bars, summer; blue bars, winter.

Article Snippet: Muscle cross‐sections (10 μm thick) were obtained using a cryostat (HM500M Microm International, Fisher Scientific, Illkirch, France) at −20°C.

Techniques: Immunofluorescence